Uttwiler Spätlauber - Swiss Apple Stem Cells – Jacqueline Piotaz Switzerland

SWISS APPLE STEM CELLS

UTTWILER SPÄTLAUBER (MALUS DOMESTICA)

Derived from a rare apple variety cultivated for its prolonged storability and moisture retention, Swiss apple stem cells offer exceptional age-defying and revitalizing effects. They can promote skin renewal, help preserve skin longevity, and reduce the appearance of fine lines and wrinkles.

EUROPEAN COSMETICS: AWARD FOR BEST ACTIVE INGREDIENT

HOW DOES IT WORK?

STUDY #1: ANTI-WRINKLE EFFECT ON CROW’S FEET

The anti-wrinkle effect of PhytoCellTec™ Malus Domestica was evaluated in a study with 20 volunteers aged from 37 to 64. An emulsion containing 2 % of PhytoCellTec™ Malus Domestica was applied twice daily for 28 days to the crow’s feet. Wrinkle depth was determined by means of PRIMOS (phase-shifting rapid in vivo measurement of skin). Results showed a significant and visible decrease in wrinkle depth for 100 % of the subjects.

S-186b / © Mibelle AG Biochemistry 2008

STUDY #2: MAINTENANCE OF STEM CELL GROWTH

An in-vitro test was conducted on umbilical cord blood stem cells with Malus Domestica stem cell extract which is the active component of PhytoCellTec™ Malus Domestica. Umbilical cord blood stem cells (UCBSC) are the “youngest” safely available stem cells for research. The influence of Malus Domestica stem cell extract on UCBSC artificial growth was evaluated by counting the cell number after incubation.

Results showed that Malus Domestica stem cell extract has a positive effect on UCBSC’s artificial growth thus maintaining the growth and the proliferative activity of UCBSC.

S-142 / © Mibelle AG Biochemistry 2008

STUDY #3: PROTECTION AGAINST UV RADIATION

A second in-vitro test was conducted on umbilical cord blood stem cells with Malus Domestica stem cell extract which is the active component of PhytoCellTec™ Malus Domestica. The protective effect against UV damage of Malus Domestica stem cell extract on UCBSC was evaluated by an MTS assay. UCBSC were incubated with different concentrations of Malus Domestica stem cell extract for 24h and were then exposed to UV radiation. The MTS assay, which measures the number of living cells and therefore the damage from UV, was performed 48h after UV radiation.

Results showed the capacity of Malus Domestica stem cell extract to protect UCBSC from UV damage even at low concentrations.

S-142 / © Mibelle AG Biochemistry 2008

STUDY #4: EFFECT ON GENE EXPRESSION IN SENESCENT (AGING) DERMAL FIBROBLASTS

Recently a cellular model for premature senescence (biological aging) was established based on normal human dermal fibroblasts. After a two hours treatment with H2O2 the cells showed the typical signs of senescence. This model was used to prove the anti-senescence effect of the Malus Domestica stem cell extract. After the H2O2 treatment, the fibroblast cells were incubated with a 2% extract for 144 hours. Then gene expression was analyzed with a cDNA array system comprising 150 probes.

Results showed that Malus Domestica stem cell extract can up-regulate specific genes involved in:

• Delay of senescence
• Protection against oxidative stress
• Repair of DNA